Solubilization of Sulfhydryl Oxidase, A Bovine Skim Milk Membrane Enzyme
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چکیده
منابع مشابه
Isolation and characterization of sulfhydryl oxidase from bovine milk.
A method is described for purification of sulfhydryl oxidase from bovine milk which consistently yields preparations with greater than 3000-fold purification over skim milk. A concentration-dependent association-dissociation of the enzyme was adapted to the development of an isolation procedure. Purified preparations exhibited two zones, both of which displayed activity, upon polyacrylamide dis...
متن کاملThe purification of a lipoprotein lipase from bovine skim milk.
The purification of a lipase from skim milk is described. The enzyme had the characteristics of a lipoprotein lipase, i.e. its activity against emulsified long chain triglyceride was stimulated more than ZO-fold by addition of suitable amounts of serum to the assay system and the activity was almost completely inhibited by 1 M NaCl. After an initial fractionation of the skim milk, the main puri...
متن کاملThe Quality of The UHT Skim Milk as Affected by Addition of Rennet Skim Milk
Background and Objectives: Consumption of whole dairy products has declined due to the awareness of possible harmful effects of fat on consumers’ health. The purpose of the present paper was to investigate the possibility of substituting the Ultra-high temperature processing (UHT) whole milk with partially hydrolyzed κ-casein to manufacture a UHT skim milk. Materials and Methods: UHT skim milk ...
متن کاملThe chemistry of xanthine oxidase. 9. An improved method of preparing the bovine milk enzyme.
Jackson, E. L. (1947). Organic Reactions, vol. 2, p. 341. New York: John Wiley and Sons Inc. Joshi, C. G. & Kulkarni, A. B. (1957). J. Indian chem. Soc. 34, 753. Keppler, H. H. (1957). J. chem. Soc. p. 2721. King, F. E. & Clark-Lewis, J. W. (1955). J. chem. Soc. p. 3384. Lillya, C. P., Drewes, S. E. & Roux, D. G. (1963a). Chem. & Ind. p. 783. Lillya, C. P., Kehoe, D., Philbin, E. M., Vickars, M...
متن کاملPreparation of monoclonal antibodies to xanthine oxidase and other proteins of bovine milk-fat-globule membrane.
Nine hybridomas secreting monoclonal antibody to proteins of bovine milk-fat-globule membrane were isolated. All nine cell lines continued to secrete monoclonal antibody after serial transfer in culture and after passage as solid tumours in Balb/cJ mice. Four of the cell lines secreted monoclonal antibody specific for xanthine oxidase, one of the major proteins of milk-fat-globule membrane.
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1982
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(82)82401-6